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An ancient split of germline and somatic stem cell lineages in Hydra

Nishimiya-Fujisawa, C.; Petersen, H.; Koubkova-Yu, T. C.-T.; Noda, C.; Shigenobu, S.; Bageritz, J.; Fujisawa, T.; Simakov, O.; Kobayashi, S.; Holstein, T. W.

2023-07-04 developmental biology
10.1101/2023.07.04.546637 bioRxiv
Show abstract

In many animals, germ cell segregation occurs during early embryogenesis to protect the genome, but its origin in basal metazoans is controversial. Here, we show in the freshwater polyp Hydra by clonal analysis and transgenic animals that interstitial stem cells comprise two separate stem cell populations, i.e., germline and multipotent somatic stem cells. We isolated genetically labelled stem cells for a global transcriptome study and discovered a broad set of germline-specific/enriched genes including Prdm9, Pax5, Dmrt1. In an alternative splicing analysis, we identified many genes with germline-specific isoforms; among them, male-specific isoforms of Dmrt1 and Snf5. The somatic interstitial stem cell lineage was characterized by numerous neuronal control genes like Neurog. But all stem cells in Hydra also share a core of stemness genes that has its roots in unicellular eukaryotes. This suggests an evolutionary scenario in which, at the emergence of animal multicellularity, there was an early split into a stable germline and different somatic stem cell lineages.

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