In vitro construction and long read sequencing analysis of a 24 kb long artificial DNA sequence encoding the Universal Declaration of the Rights of Man and of the Citizen.
Leblanc, J.; Boulle, O.; Roux, E.; Nicolas, J.; Lavenier, D.; Audic, Y.
Show abstract
In absence of DNA template, the ab initio production of long double-stranded DNA molecules of predefined sequences is particularly challenging. The DNA synthesis step remains a bottleneck for many applications such as functional assessment of ancestral genes, analysis of alternative splicing or DNA-based data storage. We propose in this report a fully in vitro protocol to generate very long double-stranded DNA molecule starting from commercially available short DNA blocks in less than 3 days. This innovative application of Golden Gate assembly allowed us to streamline the assembly process to produce a 24 kb long DNA molecule storing part of the Universal Declaration of Human rights and citizens. The DNA molecule produced can be readily cloned into suitable host/vector system for amplification and selection.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- ConSeqUMI, an error-free nanopore sequencing pipeline to identify and extract individual nucleic acid molecules from heterogeneous samples 95%
- CLUE: a bioinformatic and wet-lab pipeline for multiplexed cloning of custom sgRNA libraries 94%
- ENDO-Pore: High-throughput linked-end mapping of single DNA cleavage events using nanopore sequencing 94%
Similar papers in this journal
- C2CAplus: a one-pot isothermal circle-to-circle DNA amplification system 95%
- A Combinatorial PCR Method for Efficient, Selective Oligo Retrieval from Complex Oligo Pools 95%
- Highly parallelized construction of DNA from low-cost oligonucleotide mixtures using Data-optimized Assembly Design and Golden Gate 94%
Similar papers in this journal
- SHIMS 3.0: Highly efficient single-haplotype iterative mapping and sequencing using ultra-long nanopore reads 94%
- Nanopore Sequencing of SARS-CoV-2: Comparison of Short and Long PCR-tiling Amplicon Protocols 94%
- Amplified DNA Heterogeneity Assessment with Oxford Nanopore Sequencing Applied to Cell Free Expression Templates 93%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.