Cell-based assay to determine Type 3 Secretion System translocon assembly in Pseudomonas aeruginosa using split luciferase
Guo, H.; Geddes, E. J.; Opperman, T. J.; Heuck, A. P.
Show abstract
Multidrug resistant Pseudomonas aeruginosa poses a serious threat to hospitalized patients. This organism expresses an arsenal of virulence factors that enables it to readily establish infections and to disseminate in the host. The Type III secretion System (T3SS) and its associated effectors play a crucial role in the pathogenesis of P. aeruginosa, making them attractive targets for the development of novel therapeutic agents. The T3SS translocon, comprised of PopD and PopB, is an essential component of the T3SS secretion apparatus. In the properly assembled translocon, the N-terminus of PopD protrudes into the cytoplasm of the target mammalian cell, which can be exploited as a molecular indicator of functional translocon assembly. In this manuscript, we describe a novel whole-cell-based assay that employs the split NanoLuc luciferase detection system to provide a readout for translocon assembly. The assay demonstrates a favorable signal/noise ratio (17.9) and robustness (z=0.73), making it highly suitable for high-throughput screening of small molecule inhibitors targeting T3SS translocon assembly.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- A FRET-based high-throughput screening assay for the discovery of Mycobacterium tuberculosis DNA ADP-ribosylglycohydrolase DarG inhibitors 94%
- Evaluating the Link Between Efflux Pump Expression and Motility Phenotypes in Pseudomonas aeruginosa Treated with Virulence Inhibitors 94%
- Yersinia pestis lipopolysaccharide remodeling confers resistance to a Xenopsylla cheopis cecropin 94%
Similar papers in this journal
- Klebsiella pneumoniae type VI secretion system-mediated microbial competition is PhoPQ controlled and reactive oxygen species dependent 94%
- An NlpC/P60 protein catalyzes a key step in peptidoglycan recycling at the intersection of energy recovery, cell division and immune evasion in the intracellular pathogen Chlamydia trachomatis 93%
- Intracellular Staphylococcus aureus employs the cysteine protease staphopain A to induce host cell death in epithelial cells 93%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Establishment of a screening platform based on human coronavirus OC43 for the identification of microbial natural products with antiviral activity 94%
- The selection of antibiotic- and bacteriophage-resistant Pseudomonas aeruginosa is prevented by their combination 93%
- Novel Pentafluorosulfanyl-containing Triclocarban Analogs selectively kill Gram-positive bacteria. 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.