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A small molecule inhibitor of PTP1B and PTPN2 enhances T cell anti-tumor immunity

Liang, S.; Tran, E.; Du, X.; Dong, J.; Sudholz, H.; Chen, H.; Qu, Z.; Huntington, N.; Babon, J.; Kershaw, N.; Zhang, Z.-Y.; Baell, J.; Wiede, F.; Tiganis, T.

2023-06-16 cancer biology
10.1101/2023.06.16.545220 bioRxiv
Show abstract

The inhibition of protein tyrosine phosphatases 1B (PTP1B) and N2 (PTPN2) has emerged as an exciting approach for bolstering T cell anti-tumor immunity. ABBV-CLS-484 is a PTP1B/PTPN2 inhibitor in clinical trials for solid tumors. Here we have explored the therapeutic potential of a related small-molecule-inhibitor, Compound-182. We demonstrate that Compound-182 is a highly potent and selective active site competitive inhibitor of PTP1B and PTPN2 that enhances T cell recruitment and activation and represses the growth of tumors in mice, without promoting overt immune-related toxicities. The enhanced anti-tumor immunity in immunogenic tumors could be ascribed to the inhibition of PTP1B/PTPN2 in T cells, whereas in cold tumors, Compound-182 elicited direct effects on both tumor cells and T cells. Importantly, treatment with Compound-182 rendered otherwise resistant tumors sensitive to -PD1 therapy. Our findings establish the potential for small molecule inhibitors of PTP1B and PTPN2 to enhance anti-tumor immunity and combat cancer.

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