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mRNA vaccine induces protective immunity against the type III secretory virulence of Pseudomonas aeruginosa

Kawaguchi, K.; Kinoshita, M.; Sudo, K.; Inoue, K.; Naito, Y.; Oba, M.; Uchida, S.; Sawa, T.

2023-06-10 immunology
10.1101/2023.06.09.544431 bioRxiv
Show abstract

Few studies have addressed mRNA vaccination against bacteria despite the desperate need to control antimicrobial resistance. Herein, we developed mRNA vaccines targeting the type III secretion system of Pseudomonas aeruginosa, which plays a critical role in the pathogenesis. Vaccination with a nucleoside-modified mRNA encapsulated in lipid nanoparticles improved mouse survival, reduced the bacterial load in the lung, and alleviated pathological changes in the lungs after bacterial challenge.

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