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An intra-oral flavor discrimination task in freely moving mice

Shiotani, K.; Tanisumi, Y.; Osako, Y.; Murata, K.; Hirokawa, J.; Sakurai, Y.; Manabe, H.

2023-05-26 neuroscience
10.1101/2023.05.26.542382 bioRxiv
Show abstract

Flavor plays a critical role in the pleasure of food. Flavor research has mainly focused on human subjects and revealed that many brain regions are involved in flavor perception. However, animal models for elucidating the mechanisms of neural circuits are lacking. Herein, we demonstrate the use of a novel behavioral task in which mice are capable of flavor discrimination. When the olfactory pathways of the mice were blocked, they could not perform the task. However, behavioral accuracy was not affected when the gustatory pathway was blocked by benzocaine. These results indicate that the mice performed this discrimination task mainly based on the intraoral flavor. We conclude that this novel task can contribute to research on the neural mechanisms of flavor perception.

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