Cation permeability and pore dynamics in TRPV1 ion channels
Garcia-Avila, M.; Tello Marmolejo, J.; Rosenbaum, T.; Islas, L. D.
Show abstract
The Transient Receptor Vanilloid 1 (TRPV1) is a non-selective ion channel, which is activated by several chemical ligands and heat. We have previously shown that activation of TRPV1 by different ligands result in single-channel openings with different conductance, suggesting that the selectivity filter is highly dynamic. TRPV1 is weakly voltage-dependent, here we sought to explore whether the permeation of different monovalent ions could influence the voltage-dependence of this ion channel. By using single-channel recordings, we show that TRPV1 channels undergo rapid transitions to closed states that are directly connected to the open state, which may result from structural fluctuations of their selectivity filters. Moreover, we demonstrate that the rates of these transitions are strongly influenced by the permeant ion, suggesting that ion permeation regulates the voltage dependence of these channels.
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