Co-Expression Of Distinct Coiled Coils Can Lead To Their Entanglement
Mizgier, N. A.; Jones, C. E.; Furano, A. V.
Show abstract
L1 (LINE1) non-LTR retrotransposons are ubiquitous genomic parasites and the dominant transposable element in humans having generated about 40% of their genomic DNA during their [~]100 million years (Myr) of activity in primates. L1 replicates in early embryos, causing genetic diversity and defects, but can be active in some somatic stem cells, tumors and during aging. L1 encodes two proteins essential for retrotransposition: ORF2, a replicase, and ORF1, a coiled coil mediated homo trimer, which functions as a nucleic acid chaperone. Both proteins contain highly conserved domains and preferentially bind their encoding transcript to form an L1 ribonucleoprotein (RNP), which mediates retrotransposition. However, the coiled coil has undergone episodes of substantial amino acid replacement to the extent that a given L1 family can concurrently express multiple ORF1s that differ in the sequence of their coiled coils. Here we show that such distinct ORF1p sequences can become entangled forming heterotrimers when co-expressed from separate vectors.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Mapping the MOB proteins' proximity network reveals a unique interaction between human MOB3C and the RNase P complex 95%
- Efficient cell-free translation from diverse human cell types 95%
- A Conserved Core Region of the Scaffold NEMO is Essential for Signal-induced Conformational Change and Liquid-liquid Phase Separation 95%
Similar papers in this journal
Similar papers in this journal
- Poly-glutamine-dependent self-association as a potential mechanism for regulation of androgen receptor activity 95%
- Isoginkgetin and Madrasin are poor splicing inhibitors 94%
- Nascent mutant Huntingtin exon 1 chains do not stall on ribosomes during translation but aggregates do recruit machinery involved in ribosome quality control 93%
Similar papers in this journal
- Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits 96%
- HELZ2: a new, interferon-regulated, human 3'-5' exoribonuclease of the RNB family is expressed from a non-canonical initiation codon 96%
- Base composition at the start of the coding sequence controls the balance between translation initiation and mRNA degradation in E. coli . 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.