Analysis of in-patient evolution of Escherichia coli reveals potential links to relapse of bone and joint infections
Thiriet Rupert, S.; Josse, J.; Perez Pascual, D.; Tasse, J.; Andre, C.; Abad, L.; Lebeaux, D.; Ghigo, J.-M.; Laurent, F.; Beloin, C.
Show abstract
Bone and joint infections (BJIs) are difficult to treat and affect a growing number of patients, in which relapses are observed in 10-20% of the case. These relapses, which call for prolonged antibiotic treatment and increase the risk of emergence of resistance, may originate from ill understood adaptation of the pathogen to the host. Here, we studied three pairs of Escherichia coli strains corresponding to three cases of BJIs and their relapse to better understand in-patient adaptation. Whole genome comparison presented evidence for positive selection with prevalence of non-synonymous and loss of function mutations. Phenotypic characterization showed that biofilm formation capacity was not modified, contrary to what is usually described in such relapse cases. Although virulence was not modified, we identified the loss of two virulence factors (namely an AFA afimbrial adhesin and a YadA-like adhesin) contributing to immune system evasion in one of the studied relapse strain. Other selected strategies likely helped the relapse strains to outcompete competitors through global growth optimization and colicin production. This work highlights the variety of strategies allowing in-patient adaptation in BJIs.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Limited adaptive evolution of Staphylococcus aureus during transition from colonization to invasive infection 96%
- C-di-AMP levels modulate Staphylococcus aureus cell wall thickness as well as virulence and contribute to antibiotic resistance and tolerance 96%
- The Streptococcus agalactiae LytSR two-component regulatory system promotes vaginal colonisation and virulence in vivo 95%
Similar papers in this journal
- Capsular polysaccharides cross-regulation modulates Bacteroides thetaiotaomicron biofilm formation 96%
- NirA is an alternative nitrite reductase from Pseudomonas aeruginosa with potential as an anti-virulence target 96%
- Gentamicin induction of the gonococcal hicAB toxin-antitoxin encoding system and 2 impact on gene expression influencing biofilm formation and in vivo fitness in a strain specific manner 95%
Similar papers in this journal
- Single-cell imaging reveals that Staphylococcus aureus is highly competitive against Pseudomonas aeruginosa on surfaces 94%
- Pilot study: Trehalose-induced remodelling of the human microbiota affects Clostridioides difficile infection outcome in an in vitro colonic model 93%
- Competition among Gardnerella subgroups from the human vaginal microbiome 92%
Similar papers in this journal
- Loss of β-ketoacyl acyl carrier protein synthase III activity restores multidrug-resistant Escherichia coli sensitivity to previously ineffective antibiotics 96%
- Human Saliva Modifies Growth, Biofilm Architecture and Competitive Behaviors of Oral Streptococci 95%
- A novel conserved protein in Streptococcus agalactiae, BvaP, is important for vaginal colonization and biofilm formation 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.