B. subtilis MutS2 splits stalled ribosomesinto subunits without mRNA cleavage
Park, E.; Mackens-Kiani, T.; Berhane, R.; Esser, H.; Erdenebat, C.; Burroughs, A. M.; Berninghausen, O.; Aravind, L.; Beckmann, R.; Green, R.; Buskirk, A. R.
Show abstract
Stalled ribosomes are rescued by pathways that recycle the ribosome and target the nascent polypeptide for degradation. In E. coli, these pathways are triggered by ribosome collisions through recruitment of SmrB, a nuclease that cleaves the mRNA. In B. subtilis, the related protein MutS2 was recently implicated in ribosome rescue. Here we show that MutS2 is recruited to collisions by its SMR and KOW domains and reveal the interaction of these domains with collided ribosomes by cryo-EM. Using a combination of in vivo and in vitro approaches, we show that MutS2 uses its ABC ATPase activity to split ribosomes, targeting the nascent peptide for degradation by the ribosome quality control pathway. Notably, we see no evidence of mRNA cleavage by MutS2, nor does it promote ribosome rescue by tmRNA as SmrB cleavage does in E. coli. These findings clarify the biochemical and cellular roles of MutS2 in ribosome rescue in B. subtilis and raise questions about how these pathways function differently in various bacteria.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- A molecular switch at the yeast mitoribosomal tunnel exit controls cytochrome b synthesis 97%
- Massively parallel identification of sequence motifs triggering ribosome-associated mRNA quality control 96%
- Context-specific inhibition of mitochondrial ribosomes by phenicol and oxazolidinone antibiotics 96%
Similar papers in this journal
- PomX, a ParA/MinD ATPase activating protein, is a triple regulator of cell division in Myxococcus xanthus 96%
- Novel mechanistic insights into the role of Mer2 as the keystone of meiotic DNA break formation 96%
- Dynamic interactions between the RNA chaperone Hfq, small regulatory RNAs and mRNAs in live bacterial cells 96%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.