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descSPIM: Affordable and Easy-to-Build Light-Sheet Microscopy for Tissue Clearing Technique Users

Susaki, E. A.; Otomo, K.; Omura, T.; Nozawa, Y.; Saito, Y.

2023-05-02 bioengineering
10.1101/2023.05.02.539136 bioRxiv
Show abstract

Despite the easier use of multiple tissue clearing techniques in recent years, poor access to adequate light-sheet fluorescence microscopy remains a major obstacle for biomedical end users. Here, we propose a solution by developing descSPIM (desktop-equipped SPIM for cleared specimens) as a low-cost ($20,000-50,000), low-expertise (one-day installation by a non-expert), yet practically substantial do-it-yourself light-sheet microscopy. Academically open-sourced (https://github.com/dbsb-juntendo/descSPIM), descSPIM allows routine three-dimensional imaging of cleared samples in minutes.

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