Integrated Spatial Transcriptomic and Proteomic Analysis of Fresh Frozen Tissue Based on Stereo-seq
Liao, S.; Heng, Y.; Liu, W.; Xiang, J.; Ma, Y.; Chen, L.; Feng, X.; Jia, D.; Liang, D.; Huang, C.; Zhang, J.; Jian, M.; Su, K.; Li, M.; Loh, Y. H.; Chen, A.; Xu, X.
Show abstract
To simultaneously detect whole transcriptomes and protein markers on the same tissue section, we combined Cellular Indexing of Transcriptomes and Epitopes by Sequencing (CITE-seq) and Stereo-seq to develop the Stereo-CITE-seq workflow. Here, we demonstrated that Stereo-CITE-seq can co-detect mRNAs and proteins in immune organs with high spatial resolution, reproducibility and accuracy.
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