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Semiconducting carbon nanotubes decrease neuronal bursting in a network of rat hippocampal neurons in vitro while increasing intrinsic excitability of single neurons

Kishore, A.; Chakraborty, S.; Vasnik, S.; Ghosh, S.; Raees, M.; Sikdar, S. K.

2023-03-31 neuroscience
10.1101/2023.03.29.533880 bioRxiv
Show abstract

The diverse electrical, chemical and structural properties of the functional derivatives of carbon nanotubes (CNTs) have shown biomedical possibilities for neuroprosthesis or neural interfaces. However, the studies have been generally confined to metallic CNTs that affect cell viability unless chemically functionalized for biocompatibility. Here, we explored the effects of semiconducting single-walled carbon nanotubes (ssw-CNT), on the active electrical properties of dissociated hippocampal neurons in-vitro using multielectrode array, calcium imaging and whole-cell patch clamp recordings. The findings show that ssw-CNT treatment regulates neural network excitability from burst to tonic firing by changing the calcium dynamics. However, at a single neuronal level, ssw-CNT increases neuronal excitability.

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