Production of 130 diterpenoids by combinatorial biosynthesis in yeast
Bathe, U.; Schmidt, J.; Frolov, A.; Soboleva, A.; Frank, O.; Dawid, C.; Tissier, A. F.
Show abstract
Diterpenoids form a diverse group of natural products, many of which are or could become pharmaceuticals or industrial chemicals. However, low concentration, presence in complex mixtures and challenging synthesis often limit their exploitation. The modular character of diterpene biosynthesis and the substrate flexibility of the enzymes involved make combinatorial biosynthesis a promising approach. Here, we report on the assembly in yeast of 130 diterpenoids by pairwise combinations of ten diterpene synthases producing (+)-copalyl diphosphate-derived backbones and four cytochrome P450 enzymes (CYPs); 80 of these diterpenoids have not yet been reported. The CYPs accepted the majority of substrates they were given but remained regioselective. Our results bode well for the systematic exploration of diterpenoid chemical space using combinatorial assembly in yeast.
Matching journals
The top 9 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Widely Distributed Biosynthetic Cassette Is Responsible for Diverse Plant Side-Chain-Cross-Linked Cyclopeptides 94%
- Food-Poisoning Bacteria Employ a Citrate Synthase and a Type II NRPS to Synthesize Bolaamphiphilic Lipopeptide Antibiotics 94%
- Biosynthesis of Kaitocephalin: A Neuroprotective Natural Product Featuring a Peptide-Like yet Non-Peptidic Scaffold 93%
Similar papers in this journal
- Improved protein glycosylation enabled heterologous biosynthesis of monoterpenoid indole alkaloids and their unnatural derivatives in yeast 93%
- Transport engineering for improving production and secretion of valuable alkaloids in Escherichia coli 89%
- In vivo production of pederin by labrenzin pathway expansion 89%
Similar papers in this journal
- Early steps of the biosynthesis of the anticancer antibiotic pleurotin 95%
- Maramycin, a cytotoxic isoquinolinequinone terpenoid produced through heterologous expression of a bifunctional indole prenyltransferase /tryptophan indole-lyase in S. albidoflavus 93%
- Bioinformatic and Reactivity-Based Discovery of Linaridins 92%
Similar papers in this journal
- Engineering of critical enzymes and pathways for improved triterpenoid biosynthesis in yeast 95%
- Engineering of Phytosterol-Producing Yeast Platforms for Functional Reconstitution of Downstream Biosynthetic Pathways 94%
- The Maculalactone Biosynthetic Gene Cluster, a Cryptic Furanolide Pathway Revealed in Nodularia sp. NIES-3585 92%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.