Flexible and site-specific manipulation of histones in live animals
Finkin-Groner, E.; Al-Kachak, A.; Agustinus, A. S.; Bastle, R.; Lepack, A.; Lyu, Y.; Maze, I.; David, Y.
Show abstract
Recent advances in protein engineering have provided a wealth of methods that allow for the site-specific manipulation of proteins in vitro and in cells. However, the efforts to expand these toolkits for use in live animals has been limited. Here, we report a new method for the semi-synthesis of site-specifically modified and chemically defined proteins in live animals. Importantly, we illustrate the usefulness of this methodology in the context of a challenging, chromatin bound N-terminal histone tail within rodent postmitotic neurons located in ventral striatum (Nucleus Accumbens/NAc). This approach provides the field with a precise and broadly applicable methodology for manipulating histones in vivo, thereby serving as a unique template towards examining chromatin phenomena that may mediate transcriptomic and physiological plasticity within mammals.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Nanobody-mediated control of gene expression and epigenetic memory 95%
- Chemically modified CRISPR-Cas9 enables targeting of individual G-quadruplex and i-motif structures, revealing ligand-dependent transcriptional perturbation. 95%
- Programmable epigenome editing by transient delivery of CRISPR epigenome editor ribonucleoproteins 95%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.