Hem25p is a mitochondrial IPP transporter
Tai, J.; Guerra, R. M.; Rogers, S. W.; Fang, Z.; Muehlbauer, L. K.; Shishkova, E.; Overmyer, K. A.; Coon, J. J.; Pagliarini, D. J.
Show abstract
Coenzyme Q (CoQ, ubiquinone) is an essential cellular cofactor comprised of a redox-active quinone head group and a long hydrophobic polyisoprene tail. How mitochondria access cytosolic isoprenoids for CoQ biosynthesis is a longstanding mystery. Here, via a combination of genetic screening, metabolic tracing, and targeted uptake assays, we reveal that Hem25p--a mitochondrial glycine transporter required for heme biosynthesis--doubles as an isopentenyl pyrophosphate (IPP) transporter in Saccharomyces cerevisiae. Mitochondria lacking Hem25p fail to efficiently incorporate IPP into early CoQ precursors, leading to loss of CoQ and turnover of CoQ biosynthetic proteins. Expression of Hem25p in Escherichia coli enables robust IPP uptake demonstrating that Hem25p is sufficient for IPP transport. Collectively, our work reveals that Hem25p drives the bulk of mitochondrial isoprenoid transport for CoQ biosynthesis in yeast.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Regulation of the one carbon folate cycle as a shared metabolic signature of longevity 95%
- Discovery of EMRE in fungi resolves the true evolutionary history of the mitochondrial calciumuniporter 95%
- Beyond bacterial paradigms: uncovering the functional significance and first biogenesis machinery of archaeal lipoproteins 95%
Similar papers in this journal
- Modifications in the T arm of tRNA globally determine tRNA maturation, function and cellular fitness 94%
- Structural elucidation of the heterodimeric cis-prenyltransferase NgBR/DHDDS complex reveals novel insights in regulation of protein glycosylation 94%
- Towards CRISPR-based editing of the mitochondrial genome in yeast 94%
Similar papers in this journal
- Evolutionary conserved aspects of animal nutrient uptake and transport in sea anemone vitellogenesis 93%
- The genomes and epigenomes of aquatic plants (Lemnaceae) promote triploid hybridization and clonal reproduction 93%
- Mitochondrial function regulates cell growth kinetics to actively maintain mitochondrial homeostasis 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.