Imaging of plant calcium-sensor kinase conformation monitors real time calcium decoding in planta
Liese, A.; Eichstaedt, B.; Lederer, S.; Schulz, P.; Oehlschlaeger, J.; Feijo, J. A.; Schulze, W. X.; Konrad, K. R.; Romeis, T.
Show abstract
Changes in cytosolic calcium concentration are among the earliest reactions to a multitude of stress cues. Whereas a plethora of calcium-permeable channels may generate distinct calcium signatures and contribute to response specificities, the mechanisms by which calcium signatures are decoded is poorly understood. Here we develop a genetically encoded, FRET-based reporter that visualizes the conformational change of calcium-dependent protein kinases (CDPKs/CPKs), preceding kinase activation, for calcium-dependent AtCPK21 and calcium-independent AtCPK23. In pollen tubes, naturally displaying a physiological calcium range, CPK21-FRET, but not CPK23-FRET, report activity oscillations with similar features to cytosolic calcium, suggesting an isoform-specific calcium dependency and reversibility of the conformational change. In guard cells CPK21-FRET identifies CPK21 as a decoder of signal-specific calcium signatures in response to ABA and flg22. Based on this data, CDPK-FRET stands as a novel approach for tackling real-time live-cell calcium decoding in a multitude of plant developmental and stress responses.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- The Arabidopsis SAC9 Enzyme is enriched in a cortical population of early endosomes and restricts PI(4,5)P2 at the Plasma Membrane 96%
- Unbiased proteomic and forward genetic screens reveal that mechanosensitive ion channel MSL10 functions at ER-plasma membrane contact sites in Arabidopsis thaliana 95%
- Pectin methylesterase activity is required for RALF1 peptide signalling output 94%
Similar papers in this journal
- Plasma membrane nano-organization specifies phosphoinositide effects on Rho-GTPases and actin dynamics in tobacco pollen tubes 95%
- Constitutive activation of leucine-rich repeat receptor kinase signaling pathways by BAK1-interacting receptor-like kinase 3 chimera 95%
- Visualizing protein-protein interactions in plants by rapamycin-dependent delocalization 95%
Similar papers in this journal
Similar papers in this journal
- LORE receptor homomerization is required for 3-hydroxydecanoic acid-induced immune signaling and determines the natural variation of immunosensitivity within the Arabidopsis genus 96%
- The Ralstonia solanacearum effector RipAV targets Plant U-box proteins and induces proteasomal-dependent degradation of BIK1 94%
- Arabidopsis PROTODERMAL FACTOR2 binds lysophosphatidylcholines and transcriptionally regulates phospholipid metabolism 94%
Similar papers in this journal
- Directed growth and fusion of membrane-wall microdomains requires CASP-mediated inhibition and displacement of secretory foci 94%
- Regulation of Plant Phototropic Growth by NPH3/RPT2-like Substrate Phosphorylation and 14-3-3 Binding 94%
- The Arabidopsis NOT4A E3 ligase coordinates PGR3 expression to regulate chloroplast protein translation 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.