Continued dysfunction of capillary pericytes promotes no-reflow after experimental stroke in vivo
Shrouder, J. J.; Filser, S.; Varga, D. P.; Mamrak, U.; Besson-Girard, S.; Bulut-Impraim, B.; Seker, F. B.; Gesierich, B.; Laredo, F.; Wehn, A. C.; Khalin, I.; Bayer, P.; Liesz, A.; Gokce, O.; Plesnila, N.
Show abstract
Incomplete reperfusion of the microvasculature ("no-reflow") after ischemic stroke damages salvageable brain tissue. Previous ex-vivo studies suggest pericytes are vulnerable to ischemia and may exacerbate no-reflow, but the viability of pericytes and their association with no-reflow remains underexplored in vivo. Using longitudinal in vivo 2-photon single-cell imaging over seven days we show 87% of pericytes constrict during cerebral ischemia, remain constricted post-reperfusion and 50% of the pericyte population are acutely damaged. Moreover, we reveal ischemic pericytes are fundamentally implicated in capillary no-reflow by limiting and arresting blood flow within the first 24 hours post-stroke. Despite sustaining acute membrane damage, we observe up to 80% of cortical pericytes survive ischemia, upregulate unique transcriptomic profiles and replicate. Finally, we demonstrate delayed recovery of capillary diameter by ischemic pericytes after reperfusion predicts vessel reconstriction in the sub-acute phase of stroke. Cumulatively, these findings demonstrate surviving cortical pericytes remain both viable and promising therapeutic targets to counteract no-reflow after ischemic stroke.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Neural xenografts contribute to long-term recovery in stroke via molecular graft-host crosstalk 97%
- Capillary-associated microglia regulate vascular structure and function through PANX1-P2RY12 coupling 96%
- Pericyte-derived fibrotic scarring is conserved across diverse central nervous system lesions 96%
Similar papers in this journal
Similar papers in this journal
- Vascular Basement Membrane Laminins Modulate Functional Zonation of Cerebral Microvessels 97%
- Distinct features of brain perivascular fibroblasts and mural cells revealed by in vivo two-photon imaging 95%
- Assessing post-stroke cognition in pre-clinical models: lessons and recommendations from a multi-center study 94%
Similar papers in this journal
- The myeloid cell-driven transdifferentiation of endothelial cells into pericytes promotes the restoration of BBB function and brain self-repair after stroke 97%
- T cells modulate the microglial response to brain ischemia 95%
- Subventricular zone/white matter microglia reconstitute the empty adult microglial niche in a dynamic wave 94%
Similar papers in this journal
- Spatiotemporal transcriptomic maps of mouse intracerebral hemorrhage at single-cell resolution 95%
- Brain-Engrafted Monocyte-derived Macrophages from Blood and Skull-Bone Marrow Exhibit Distinct Identities from Microglia 93%
- Microglia are not required for maintenance of blood-brain barrier properties in health, but PLX5622 alters brain endothelial cholesterol metabolism 92%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.