The commensal protist Tritrichomonas musculus exhibits a dynamic life cycle that induces B cell-modulated remodeling of the gut microbiota
Popovic, A.; Cao, E. Y.; Han, J.; Nursimulu, N.; Alves-Ferreira, E. V. C.; Kennard, A.; Alsmadi, N.; Grigg, M. E.; Mortha, A.; Parkinson, J.
10.1101/2023.03.06.528774 bioRxivShow abstract
Commensal protists and gut bacterial communities exhibit complex relationships, mediated at least in part through host immunity. To improve our understanding of this tripartite interplay, we investigated community and functional dynamics between the murine protist Tritrichomonas musculus (T. mu) and intestinal bacteria in healthy and B cell-deficient mice. We identified dramatic, protist-driven remodeling of resident microbiome growth and activities, in parallel with T. mu functional changes, accelerated in the absence of B cells. Metatranscriptomic data revealed nutrient-based competition between bacteria and the protist. Single cell transcriptomics identified distinct T. mu life stages, providing new evidence for trichomonad sexual replication and the formation of pseudocysts. Unique cell states were validated in situ through microscopy and flow cytometry. Our results reveal complex microbial dynamics during the establishment of a commensal protist in the gut, and provide valuable datasets to drive future mechanistic studies.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Blood-borne immune cells carry low biomass DNA remnants of microbes in patients with colorectal cancer or inflammatory bowel disease 95%
- MRGM: An enhanced catalog of mouse gut microbial genomes substantially broadening taxonomic and functional landscapes 95%
- The influence of early life exposures on the infant gut virome 94%
Similar papers in this journal
- Clostridia isolated from helminth-colonized humans promote hatching of Trichuris muris 94%
- Antibiotic perturbation of the human gut phageome preserves its individuality and promotes blooms of virulent phages. 94%
- Dynamic modulation of spleen germinal center reactions by gut bacteria during Plasmodium infection 94%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.