Decoding Duchenne muscular dystrophy transcriptome to single nuclei level reveals clinical-genetic correlations
Suarez-Calvet, X.; Fernandez-Simon, E.; Natera, D.; Jou, C.; Pinol-Jurado, P.; Villalobos, E.; Ortez, C.; Monceau, A.; Schiava, M.; Verdu-Diaz, J.; Clark, J.; Laidler, Z.; Mehra, P.; Gokul-Nath, R.; Alonso-Perez, J.; Marini-Bettolo, C.; Tasca, G.; Straub, V.; Guglieri, M.; Nascimento, A.; Diaz-Manera, J.
Show abstract
The cellular and molecular consequences of lack of dystrophin in humans are only partially known, which is crucial for the development of new therapies aiming to slow or stop the progression Duchenne and Becker muscular dystrophies. We analyzed muscle biopsies of DMD patients and controls using single nuclei RNA sequencing (snRNAseq) and correlated the results with clinical data. DMD samples displayed an increase in regenerative fibers, satellite cells and fibro-adipogenic progenitor cells (FAPs) and a decrease in slow fibers and smooth muscle cells. Samples from patients with stable mild weakness were characterized by an increase in regenerative fibers, while those from patients with progressive weakness had fewer muscle fibers and increased FAPs. DMD muscle fibers displayed a strong regenerative signature, while DMD FAPs upregulated genes producing extracellular matrix and molecules involved in several signaling pathways. An analysis of intercellular communication profile identified FAPs as a key regulator of cell signaling in DMD samples. We show significant differences in the gene expression profiled of the different cell populations present in DMD muscle samples compared to controls.
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