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CD8+ cells and small viral reservoirs facilitate post-ART control of SIV in Mauritian cynomolgus macaques

Harwood, O. E.; Matschke, L. M.; Moriarty, R. V.; Balgeman, A. J.; Weaver, A. J.; Ellis-Connell, A. L.; Weiler, A. M.; Winchester, L. C.; Fletcher, C. V.; Friedrich, T.; Keele, B. F.; O'Connor, D. H.; Lang, J. D.; Reynolds, M. R.; O'Connor, S. L.

2023-03-02 immunology
10.1101/2023.03.01.530655 bioRxiv
Show abstract

Sustainable HIV remission after antiretroviral therapy (ART) withdrawal, or post-treatment control (PTC), remains a top priority for HIV treatment. We observed surprising PTC in an MHC-haplomatched cohort of MHC-M3+ SIVmac239+ Mauritian cynomolgus macaques (MCMs) initiated on ART at two weeks post-infection (wpi). For six months after ART withdrawal, we observed undetectable or transient viremia in seven of eight MCMs. In vivo depletion of CD8+ cells induced rebound in all animals, indicating the PTC was mediated, at least in part, by CD8+ cells. We found that MCMs had smaller acute viral reservoirs than a cohort of identically infected rhesus macaques, a population that rarely develops PTC. The mechanisms by which unusually small viral reservoirs and CD8+ cell-mediated virus suppression enable PTC can be investigated using this MHC-haplomatched MCM model. Further, defining the immunologic mechanisms that engender PTC in this model may identify therapeutic targets for inducing durable HIV remission in humans.

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