Time course of lipopolysaccharide- and capsaicin-induced cytokine release in an ex vivo mouse model of vaginal inflammation
Chalmers, K. J.; Hutchinson, M. R.; Dodds, K. N.; Kwok, Y. H.; Evans, S. F.; Moseley, G. L.
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ProblemThe neuroimmune interface has been characterised in few areas of the body. The objective of this study was to investigate the neuroimmune interface in the mouse vagina through a novel ex vivo model, to determine whether LPS could directly activate and produce TRPV1-mediated neuronal activation. Method of StudyConcentrations of IL-1{beta} and IL-6 release into the supernatant at different times post ex vivo stimulation with LPS, capsaicin, or a combination of the two were assessed using enzyme-linked immunosorbent assay. ResultsThere were no differences in the supernatant concentration of IL-6 with different stimulation type nor stimulation time. Supernatant concentrations of IL-1{beta} were greater at the 20 hour time point than the 4 hour time point, and were greater for stimulations involving LPS. ConclusionThere is a clear pattern of pro-inflammatory neuroimmune responses following ex vivo stimulation of mouse vaginal tissues with capsaicin and LPS, evident as an increased IL-1{beta} output. This output is greatest at 20 hours post-stimulation, indicating this neuroimmune response is time-dependent.
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