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Altered IL-6 signalling and risk of tuberculosis disease: a meta-analysis and Mendelian randomisation study

Hamilton, F.; Schurz, H.; Yates, T. A.; Gilchrist, J. W.; Moller, M.; Naranbhai, V.; Ghazal, P.; Timpson, N. J.; International Host TB Genetics Consortium, ; Parks, T.; Pollara, G.

2023-02-08 infectious diseases
10.1101/2023.02.07.23285472 medRxiv
Show abstract

IL-6 responses are ubiquitous in Mycobacterium tuberculosis (Mtb) infections, but their role in determining human tuberculosis (TB) disease risk is unknown. We used single nucleotide polymorphisms (SNPs) in and near the IL-6 receptor (IL6R) gene, focusing on the non-synonymous variant, rs2228145, associated with reduced classical IL-6 signalling, to assess the effect of altered IL-6 activity on TB disease risk. We identified 16 genome wide association studies (GWAS) of TB disease collating 17,982 cases of TB disease and 972,389 controls across 4 continents. Meta-analyses and Mendelian randomisation analyses revealed that reduced classical IL-6 signalling was associated with lower odds of TB disease, a finding replicated using multiple, independent SNP instruments and 2 separate exposure variables. Our findings establish a causal relationship between IL-6 signalling and the outcome of Mtb infection, suggesting IL-6 antagonists do not increase the risk of TB disease and should be investigated as adjuncts in treatment.

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