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The ThCre:mTmG mouse has sparse expression in the sympathetic nervous system

Tian, T.; Ward, P. J.

2023-02-03 neuroscience
10.1101/2023.02.01.526373 bioRxiv
Show abstract

The global double-fluorescent cre-dependent reporter mouse mT/mG has been a valuable tool for differential identification of tissues and cells. This line was previously shown to have EGFP expression in a cre-dependent fashion in single neurons. Here, we demonstrate very sparse labeling in a ThCre:mT/mG cross. This is evident in sweat glands, thoracic spinal cord, sciatic nerve, and lumbar sympathetic ganglia. Therefore, this model may be suitable for imaging or electrophysiology studies of postganglionic sympathetic neuron cell bodies but ineffective for visualizing peripheral axons and tissue targets of the sympathetic nervous system.

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