ChIP-MS reveals the local chromatin composition by label-free quantitative proteomics
Yong, W. K.; Rane, G.; Zaal Anuar, N.; Shao, X.; Goh, C. Y.; Khanchandani, V.; Kuan, V. L. S.; Jeitany, M.; Koeffler, P.; Deng, L.-W.; Sanda, T.; Kappei, D.
Show abstract
Chromatin immunoprecipitation (ChIP) has been a cornerstone for epigenetic analyses over the last decades, but even coupled to sequencing approaches (ChIP-seq), it is ultimately limited to one protein at a time. In a complementary effort, we here combined ChIP with label-free quantitative (LFQ) mass spectrometry (ChIP-MS) to interrogate local chromatin compositions. We demonstrate the versatility of our approach at telomeres, with transcription factors, in tissue and by dCas9-driven locus-specific enrichment.
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