Bioengineering of a Lactococcus lactis subsp. lactis strain enhances nisin production and bioactivity.
Portieles, R.; Xu, H.; Chen, F.; Gao, J.; Du, C.; Gao, X.; Nordelo, C. B.; Yue, Q.; Zhao, L.; Gonzalez, N. P.; Bermudez, R. S.; Borras-Hidalgo, O.
Show abstract
Lactococcus lactis subsp. lactis is a food bacterium that has been utilized for decades in food fermentation and the development of high-value industrial goods. Among these, nisin is produced by several strains of L. lactis subsp. lactis plays a crucial role as a food bio-preservative. The relative expression of the gene cluster involved in nisin production was evaluated using qPCR analysis. Additionally, a series of re-transformations of the strain introducing multi copies of the nisA and nisRK genes involved in nisin biosynthesis were developed. The simultaneous expression of nisA and nisZ genes was used to potentiate the effective inhibition of foodborne pathogens. Further, qPCR analysis revealed a low expression of the nisA and nisRK genes in wild-type L. lactis subsp. lactis. After several re-transformations of the strain with the nisA and nisRK genes, a high expression of these genes was obtained, contributing to improved nisin production. Also, the co-expression of nisA and nisZ genes results in highly efficient antimicrobial activity. Hence, this study would supply an approach to enhancing nisin production during industrial processes and antimicrobial activity.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cloning and functional expression of a food-grade circular bacteriocin, plantacyclin B21AG, in probiotic Lactobacillus plantarum WCFS1 97%
- Characterization of the Corynebacterium glutamicum dehydroshikimate dehydratase QsuB and its potential for microbial production of protocatechuic acid 96%
- High-yield Production of Recombinant Platelet Factor 4 Protein by Harnessing and Honing the Gram-negative Bacterial Secretory Apparatus 95%
Similar papers in this journal
- Quorum Sensing Regulators and Non-ribosomal Peptide Synthetases Govern Antibacterial Secretions in Xenorhabdus szentirmaii 96%
- Listening to bacterial Esperanto: transcriptome reprogramming in a plant beneficial rhizobacterium 95%
- Restriction of the growth and biofilm formation of ESKAPE pathogens by caprine gut-derived probiotic bacteria 95%
Similar papers in this journal
- Tailoring and optimizing fatty acid production by oleaginous yeasts through the systematic exploration of their physiological fitness 95%
- Metabolic engineering of Escherichia coli for optimized biosynthesis of nicotinamide mononucleotide, a noncanonical redox cofactor 95%
- H2-driven xylitol production in Cupriavidus necator H16 93%
Similar papers in this journal
- (p)ppGpp and DksA play crucial role in reducing the efficacy of b-lactam antibiotics by modulating bacterial membrane permeability 95%
- Quantitative physiology and proteome adaptations of Bifidobacterium breve NRBB57 at near-zero growth rates 95%
- Comparative Genomic Analysis of Metal-Tolerant Bacteria Reveals Significant Differences in Metal Adaptation Strategies 94%
Similar papers in this journal
- Catabolism of 3-hydroxypyridine by Ensifer adhaerens HP1: a novel four-component gene encoding 3-hydroxypyridine dehydrogenase HpdA catalyzes the first step of biodegradation 96%
- Multistep metabolic engineering of Bacillus licheniformis to improve pulcherriminic acid production 96%
- Paenibacillus sp. strain UY79, isolated from a root nodule of Arachis villosa, displays a broad spectrum of antifungal activity 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.