Apical size and deltaA expression predict adult neural stem cell decisions along lineage progression
Mancini, L.; Guirao, B.; Ortica, S.; Labusch, M.; Cheysson, F.; Bonnet, V.; Phan, M. S.; Herbert, S.; Mahou, P.; Menant, E.; Bedu, S.; Tinevez, J.-Y.; Baroud, C.; Beaurepaire, E.; Bellaiche, Y.; Bally-Cuif, L.; Dray, N.
Show abstract
The maintenance of neural stem cells (NSCs) in the adult brain depends on their activation frequency and division mode. We use long-term intravital imaging of NSCs in the zebrafish adult telencephalon to link activation and division mode with predictive cellular and molecular parameters. We reveal that apical surface area and expression of the Notch ligand DeltaA predict NSC activation frequency, while deltaA expression marks NSC commitment to neurogenesis. We also find that deltaA-negative NSCs constitute the bona fide self-renewing NSC pool and systematically engage in asymmetric divisions generating a self-renewing deltaAneg and a neurogenic deltaApos NSC. Finally, modulation of Notch signaling during imaging indicates that the prediction of activation frequency by apical size, and the asymmetric divisions of deltaAneg NSCs, are functionally independent of Notch. These results provide dynamic qualitative and quantitative readouts of NSC lineage progression in vivo and support a hierarchical organization of NSCs in differently fated sub-populations.
Matching journals
The top 3 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Cellular and transcriptional trajectories of neural fate specification in sea anemone uncover two modes of adult neurogenesis 97%
- Integrating central and peripheral neurons in elongating multi-lineage-organized gastruloids 96%
- Loss of PRC2 subunits primes lineage choice during exit of pluripotency 96%
Similar papers in this journal
- Variation of human neural stem cells generating organizer states in vitro before committing to cortical excitatory or inhibitory neuronal fates 96%
- Single-cell profiling and SCOPE-seq reveal the lineage dynamics of adult neurogenesis and NOTUM as a key V-SVZ regulator 95%
- Dual nuclease single-cell lineage tracing by Cas9 and Cas12a 95%
Similar papers in this journal
Similar papers in this journal
- Lineage hierarchies and stochasticity ensure the long-term maintenance of adult neural stem cells 97%
- Integrated single-cell transcriptomic and epigenetic analyses of cell-state transition and lineage commitment in the embryonic mouse cerebellum 96%
- A telencephalon cell type atlas for goldfish reveals diversity in the evolution of spatial structure and cell types 96%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.