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Superinfection plays an important role in the acquisition of complex Plasmodium falciparum infections among female Anopheles mosquitoes

Berube, S.; Freedman, B.; Menya, D.; Kipkoech, J.; Abel, L.; Lapp, Z.; Taylor, S. M.; O'Meara, W. P.; Obala, A. A.; Wesolowski, A.

2022-12-27 systems biology
10.1101/2022.12.23.521802 bioRxiv
Show abstract

Studies of human malaria infections with multiple, genetically distinct parasites have illuminated mechanisms of malaria transmission. However, few studies have used the genetic diversity in mosquito infections to understand how transmission is sustained. We identified likely human sources of mosquito infections from a longitudinal cohort in Western Kenya based on genetic similarity between parasites and the timing of infections. We found that several human infections were required to reconstitute each mosquito infection and that multiple parasite clones were likely transmitted from infected humans to mosquitoes in each bite, suggesting that superinfection and co-transmission occur simultaneously and are important mechanisms of transmission. We further investigated this using an individual human and mosquito simulation model and found that co-transmission alone was unlikely to reproduce the high complexity of mosquito infections. We concluded that the superinfection of mosquitoes likely plays an important, but under studied, role in sustaining moderate to high malaria transmission.

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