HIV-1 diverts actin debranching mechanismsfor particle assembly and release in CD4 Tlymphocytes.
DIBSY, R.; Bremaud, E.; MAK, J.; Favard, C.; MURIAUX, D.
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Enveloped viruses assemble and bud from the host cell membranes. Possible roles of cortical actin in these processes have often been a source of controversy. Here, we assessed the involvement of the Arp2/3 mediated branched actin in HIV-1 assembly at the membrane of infected CD4 T lymphocytes. Our results show that actin debranching not only increases HIV-1 release but also the number of individual HIV-1 assembly clusters present at the cell plasma membrane unravelling new mechanisms. Indeed, we showed that, in infected T lymphocytes, HIV-1 Gag prefers areas deficient in F-actin for assembly. In vitro, we could reproduce and quantify this mechanism using model systems. Finally, we found that the actin debranching factor, Arpin, an Arp2/3 inhibitor, is recruited by Gag at the cell membrane to promote virus assembly. Altogether, our data show that HIV-1 favors local actin debranching for assembly and release by subverting the host factor Arpin.
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