Acceleration of genome replication uncovered by single-cell nascent DNA sequencing
van den Berg, J.; van Batenburg, V.; van Oudenaarden, A.
Show abstract
In a human cell thousands of replication forks simultaneously coordinate the duplication of the entire genome. The rate at which this process occurs, might depend on the epigenetic state of the genome and vary between, or even within, cell types. To accurately measure DNA replication speeds, we developed a technology to detect recently replicated DNA using single-cell sequencing. Replication speed is not constant but increases during S-phase of the cell cycle. Using genetic and pharmacological perturbations we are able to alter this acceleration of replication and conclude that DNA damage inflicted by the process of transcription limits the speed of replication during early S-phase. In late S-phase, during which less transcription occurs, replication accelerates and approaches its maximum speed.
Matching journals
The top 1 journal accounts for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Single-molecule tracking reveals two low-mobility states for chromatin and transcriptional regulators within the nucleus 96%
- Relief of ParB autoinhibition by parS DNA catalysis and ParB recycling by CTP hydrolysis promote bacterial centromere assembly. 96%
- Coordinated DNA and Histone Dynamics Drive Accurate Histone H2A.Z Exchange 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.