The fast diffusion of NKCC1 along the axon is driven by glutamatergic activity
Come, E.; Pol, E.; Merlaud, Z.; Gouhier, J.; Russeau, M.; MARQUES, X.; Scotto-Lomassese, S.; Moutkine, I.; LEVI, S.
Show abstract
NKCC1 and KCC2 transporters regulate neuronal chloride homeostasis and thus synaptic inhibition. KCC2 activity is tuned by diffusion-capture in an activity-dependent manner. The mechanisms controlling NKCC1 in neurons are unknown. We found using super-resolution imaging that NKCC1 like KCC2 form nanodomains in the somato-dendritic membrane at extrasynaptic sites and at the periphery of excitatory and inhibitory synapses. NKCC1 nanoclusters are half the size and density in molecules than KCC2 clusters. This is accompanied by a higher mobility of NKCC1 compared to KCC2 in the dendritic membrane, suggesting a weaker NKCC1 anchoring to the cytoskeleton. In contrast, NKCC1, but not KCC2, is confined to endocytic zones, which would explain its controlled surface expression and the fact that endocytic zones would provide a reservoir from which NKCC1 could be released into the membrane. Finally, we show an increased confinement of NKCC1 in axons but not in dendrites upon glutamatergic activity blockade, indicating a selective mechanism of regulation in the axon. We propose that a rapid regulation of NKCC1 by lateral diffusion in the axon would control presynaptic glutamate release and the firing of action potentials.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Dynamics and nanoscale organization of the postsynaptic endocytic zone at excitatory synapses 97%
- Syntaxin-1A modulates vesicle fusion in mammalian neurons via juxtamembrane domain dependent palmitoylation of its transmembrane domain 96%
- Rapid recycling of glutamate transporters on the astroglial surface 96%
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- Postsynaptic neuroligin-1 mediates presynaptic endocytosis during neuronal activity 95%
- Nanoscopical analysis reveals an orderly arrangement of the presynaptic scaffold protein Bassoon at the Golgi-apparatus 94%
- Distinct spatial distribution of potentiated dendritic spines in encoding- and recall-activated hippocampal neurons 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.