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Bulk and Mosaic Deletions of Egfr Reveal Regionally Defined Gliogenesis in the Developing Mouse Forebrain

Zhang, X.; Xiao, G.; Johnson, C. A.; Cai, Y.; Horowitz, Z.; Mennicke, C.; Coffey, R.; Haider, M.; Threadgill, D. W.; Eliscu, R.; Oldham, M. C.; Greenbaum, A. C.; Ghashghaei, H. T.

2022-12-11 neuroscience
10.1101/2022.12.10.519926 bioRxiv
Show abstract

The Epidermal growth factor receptor (EGFR) plays a role in cell proliferation and differentiation during healthy development and tumor growth, however its requirement for brain development remains unclear. Here we used a conditional mouse allele for Egfr to examine its contributions to perinatal forebrain development at the tissue level. Subtractive bulk ventral and dorsal forebrain deletions of Egfr uncovered significant and permanent decreases in oligodendrogenesis and myelination in the cortex and corpus callosum. Additionally, an increase in astrogenesis or reactive astrocytes in effected regions was evident in response to cortical scarring. Sparse deletion using Mosaic Analysis with Double Markers (MADM) surprisingly revealed a regional requirement for EGFR in rostrodorsal, but not ventrocaudal glial lineages including both astrocytes and oligodendrocytes. The EGFR-independent ventral glial progenitors may compensate for the missing EGFR-dependent dorsal glia in the bulk Egfr-deleted forebrain, potentially exposing a regenerative population of gliogenic progenitors in the mouse forebrain.

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