Genetically encoded multimeric tags for intracellular protein localisation in cryo-EM
Fung, H. K.; Hayashi, Y.; Salo, V. T.; Babenko, A.; Zagoriy, I.; Brunner, A.; Ellenberg, J.; Mueller, C. W.; Cuylen-Haering, S.; Mahamid, J.
10.1101/2022.12.10.519870 bioRxivShow abstract
Cryo-electron tomography is a powerful label-free tool for visualizing biomolecules in their native cellular context at molecular resolution. However, the precise localisation of biomolecules of interest in the tomographic volumes is challenging. Here, we present a tagging strategy for intracellular protein localisation based on genetically encoded multimeric particles (GEMs). We show the applicability of drug-controlled GEM labelling of endogenous proteins in cryo-electron tomography and cryo-correlative fluorescence imaging in human cells.
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