8-HQA Adjusts the Number and Diversity of Bacteria in the Gut Microbiome of Spodoptera littoralis
Mazumdar, T.; Haenniger, S.; Shukla, S.; Murali, A.; Bartram, S.; Heckel, D. G.; Boland, W.
Show abstract
Quinolinic carboxylic acids are known for their metal ion chelating properties in insects, plants and bacteria. The larval stages of the lepidopteran pest, Spodoptera littoralis, produce 8-hydroxyquinoline-2-carboxylic acid (8-HQA) in high concentrations from tryptophan in the diet. At the same time, the larval midgut is known to harbor a bacterial population. The motivation behind the work was to investigate whether 8-HQA is controlling the bacterial community in the gut by regulating the concentration of metal ions. Knocking out the gene for kynurenine 3-monooxygenase (KMO) in the insect using CRISPR/Cas9 eliminated production of 8-HQA and significantly increased bacterial numbers and diversity in the larval midgut. Adding 8-HQA to the diet of knockout larvae caused a dose-dependent reduction of bacterial numbers with minimal effects on diversity. Enterococcus mundtii dominates the community in all treatments, probably due to its highly efficient iron uptake system and production of the colicin, mundticin. Thus host factors and bacterial properties interact to determine patterns of diversity and abundance in the insect midgut.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Gut microbiota of Drosophila subobscura contributes to its heat tolerance but is sensitive to transient thermal stress 95%
- Dietary emulsifiers alter composition and activity of the human gut microbiota in vitro, irrespective of chemical or natural emulsifier origin. 92%
- Experimental warming reduces gut prokaryotic diversity, survival and thermal tolerance of the eastern subterranean termite, Reticulitermes flavipes (Kollar) 92%
Similar papers in this journal
- Cuticular hydrocarbon biosynthesis in malaria vectors: insights from the adult oenocyte transcriptome 94%
- A beta-glucosidase of an insect herbivore determines both toxicity and deterrence of a dandelion defense metabolite 94%
- A gustatory receptor tuned to the steroid plant hormone brassinolide in Plutella xylostella (Lepidoptera: Plutellidae) 93%
Similar papers in this journal
Similar papers in this journal
- Pleiotropic role of PAX cyclolipopeptides in the Xenorhabdus bacterium mutualistically associated with entomopathogenic nematodes 96%
- Zooplankton as a transitional host for Escherichia coli in freshwater 94%
- Microbial Associates of the Elm Leaf Beetle: Uncovering the Absence of Resident Bacteria and the Influence of Fungi on Insect Performance 93%
Similar papers in this journal
- Influence of the lab adopted natural diet and environmental and parental microbiota on life history and metabolic phenotype of Drosophila melanogaster larvae. 95%
- RNase R, a new virulence determinant of Streptococcus pneumoniae 93%
- Exploring the phytobeneficial and biocontrol capacities of endophytic bacteria isolated from hybrid vanilla pods 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.