Toward a Cell Culture Model of Portal Axis Lipid Handling
Cook, J. R.; Green, P. H. R.; Haeusler, R. A.
Show abstract
The health risks posed by excessive visceral white adipose tissue (WAT) may arise from exposure to deleterious intestinal factors in transit through the portal system. These may include an altered bile acid (BA) pool - particularly with respect to 12-hydroxylated BA (12-HBA), which we have previously demonstrated are closely associated with insulin resistance. However, given the complexity of the enterohepatic milieu, we have worked to develop a co-culture system that allows for controlled study of the interactions between human induced pluripotent stem cell (hiPSC) derived adipocytes and primary human intestinal organoids (hIO). We present preliminary data on the characterization of both cellular compartments and their interactions, particularly in the context of BA treatment. We have recapitulated the basic functionality of each tissue type and have found reciprocal, potentially important changes in gene expression in each. However, we also have encountered numerous technical challenges in the development of this co-culture system and provide methodological observations to assist others seeking to work with a co-culture system of this type.
Matching journals
The top 10 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- SCD1 and monounsaturated lipids are required for autophagy and survival of adipocytes 95%
- Adipocyte-specific ablation of the Ca2+ pump SERCA2 impairs whole-body metabolic function and reveals the diverse metabolic flexibility of white and brown adipose tissue. 94%
- Salt-inducible kinase inhibition promotes the adipocyte thermogenic program and adipose tissue browning 94%
Similar papers in this journal
- Isolation of adipose tissue derived regenerative cells from human subcutaneous tissue with or without the use of an enzymatic reagent 95%
- MG53 is not a critical regulator of insulin signaling pathway in skeletal muscle 94%
- Core circadian clock transcription factor BMAL1 regulates mammary epithelial cell growth, differentiation, and milk component synthesis 93%
Similar papers in this journal
- Raman microspectroscopy reveals unsaturation heterogeneity at the lipid droplet level and validates an in vitro model of bone marrow adipocyte subtypes 95%
- Thiamine transporter 2 and Janus kinase 2 inhibitor, fedratinib suppresses thermogenic activation of human neck area-derived adipocytes 93%
- GLP-1 Receptor Agonist Improves Metabolic Disease in a Pre-clinical Model of Lipodystrophy 93%
Similar papers in this journal
- Emodin-Enhanced hUC-MSC Extracellular Vesicles Alleviate Acute Pancreatitis by Targeting Inflammation and Pyroptosis 93%
- Drug metabolism activity is a Critical Intrinsic Selector for Hepatocytes to elongate cell lifespan by using a cell-killing antibiotic 93%
- Novel therapeutic strategies for injured endometrium: Autologous intrauterine transplantation of menstrual blood-derived cells from infertile patients 92%
Similar papers in this journal
- Matrix Metalloproteinase-2 as a novel regulator of glucose utilization by adipocytes 93%
- Novel insights into the regulation of chemerin expression: role of acute-phase cytokines and DNA methylation 93%
- Identification and implication of tissue-enriched ligands in epithelial-endothelial crosstalk during pancreas development 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.