Proteomic Analysis Defines the Interactome of Telomerase in the Protozoan Parasite, Trypanosoma brucei
Davis, J. A.; Reyes, A. V.; Nitika, ; Saha, A.; Wolfgeher, D. J.; Xu, S.-L.; Truman, A.; Li, B.; Chakrabarti, K.
Show abstract
Telomerase is a ribonucleoprotein enzyme responsible for maintaining the telomeric end of the chromosome. The telomerase enzyme requires two main components to function: the telomerase reverse transcriptase (TERT) and the telomerase RNA (TR), which provides the template for telomeric DNA synthesis. TR is a long noncoding RNA, which forms the basis of a large structural scaffold upon which many accessory proteins can bind and form the complete telomerase holoenzyme. These accessory protein interactions are required for telomerase activity and regulation inside cells. The interacting partners of TERT have been well studied in yeast, human, and Tetrahymena models, but not in lower eukaryotes, including clinically relevant human parasites. Here, using the protozoan parasite, Trypanosoma brucei (T. brucei) as a model, we have identified the interactome of T. brucei TERT (TbTERT) using a mass spectrometry-based approach. We identified previously known and unknown interacting factors of TbTERT, highlighting unique features of T. brucei telomerase biology. These unique interactions with TbTERT, suggest mechanistic differences in telomere maintenance between T. brucei and other eukaryotes.
Matching journals
The top 12 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Novel axonemal protein ZMYND12 interacts with TTC29 and DNAH1, and is required for male fertility and flagellum function. 94%
- The SPARC complex defines RNAPII promoters in Trypanosoma brucei 93%
- A plant-like mechanism coupling m6A reading to polyadenylation safeguards transcriptome integrity and developmental genes partitioning in Toxoplasma 93%
Similar papers in this journal
- Immunoprecipitation of RNA-DNA hybrid interacting proteins in Trypanosoma brucei reveals conserved and novel activities, including in host immune evasion by antigenic variation 95%
- Genome maintenance functions of Trypanosoma brucei DNA Polymerase N include telomere association and a role in antigenic variation 93%
- A termination-independent role of Rat1 in cotranscriptional splicing 93%
Similar papers in this journal
- Translational control by Trypanosoma brucei DRBD18 contributes to the maintenance of the procyclic state 94%
- The kinesin motor KIF1C is a putative transporter of the exon junction complex in neuronal cells 93%
- Multiple domains of the integral KREPA3 protein are critical for the structure and precise functions of RNA Editing Catalytic Complexes in Trypanosoma brucei 93%
Similar papers in this journal
- Metabolic selection of a homologous recombination mediated loss of glycosomal fumarate reductase in Trypanosoma brucei 92%
- Interactome analysis of C. elegans synapses by TurboID-based proximity labeling 91%
- An improved TurboID pipeline in C. elegans by biochemical depletion of endogenously biotinylated carboxylases 91%
Similar papers in this journal
- Meiotic DNA breaks activate a streamlined phospho-signaling response that largely avoids protein level changes 92%
- Loss-of-function cancer-associated mutations in the EIF4G2 non-canonical translation initiation factor 91%
- Stress-induced tyrosine phosphorylation of RtcB modulates IRE1 activity and signaling outputs. 91%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.