Back

Split selectable marker mediated gene stacking in plants

Yuan, G.; Lu, H.; De, K.; Hassan, M. M.; Wellington, M.; Tuskan, G. A.; Yang, X.

2022-11-20 plant biology
10.1101/2022.11.19.517202 bioRxiv
Show abstract

Plant synthetic biology and genetic engineering depend on the controlled expression of transgenes of interest, which relies heavily on the use of Agrobacterium-mediated transformation. Here we establish a novel split selectable marker system using protein splicing elements called "inteins" for Agrobacterium-mediated co-transformation in plants. This method enables robust co-transformation in Arabidopsis and poplar, providing a novel strategy for the simultaneous insertion of multiple genes into both herbaceous and woody plants.

Matching journals

The top 4 journals account for 50% of the predicted probability mass.

1
Nature Plants
○ 94 papers in training set
Top 0.1%
21.5%
Similar papers in this journal
2
Plant Biotechnology Journal
○ 64 papers in training set
Top 0.1%
14.8%
Similar papers in this journal
3
Molecular Plant
○ 39 papers in training set
Top 0.1%
9.5%
Similar papers in this journal
4
Nature Communications
○ 5641 papers in training set
Top 24%
6.6%
Similar papers in this journal
50% of probability mass above
5
Scientific Reports
○ 3612 papers in training set
Top 16%
5.4%
Similar papers in this journal
6
ACS Synthetic Biology
● 287 papers in training set
Top 0.8%
4.0%
7
eLife
● 5828 papers in training set
Top 36%
3.2%
8
Plant Communications
○ 36 papers in training set
Top 0.2%
3.2%
9
Plant Physiology
◐ 238 papers in training set
Top 2%
2.4%
10
The Plant Journal
○ 215 papers in training set
Top 2%
2.3%
11
Horticulture Research
◐ 47 papers in training set
Top 0.4%
2.1%
12
The CRISPR Journal
○ 39 papers in training set
Top 0.2%
2.1%
13
New Phytologist
○ 346 papers in training set
Top 3%
1.9%
14
Frontiers in Plant Science
○ 256 papers in training set
Top 3%
1.7%
15
Journal of Integrative Plant Biology
○ 13 papers in training set
Top 0.2%
1.7%
16
PLOS ONE
● 5266 papers in training set
Top 48%
1.7%
17
The Plant Cell
◐ 161 papers in training set
Top 2%
1.4%
18
Communications Biology
○ 993 papers in training set
Top 19%
1.3%
19
DNA Research
◐ 26 papers in training set
Top 0.3%
1.0%
20
Proceedings of the National Academy of Sciences
● 2444 papers in training set
Top 39%
1.0%
21
Current Biology
○ 665 papers in training set
Top 10%
0.8%
22
Plant and Cell Physiology
◐ 52 papers in training set
Top 2%
0.6%
23
Molecular Biology and Evolution
◐ 542 papers in training set
Top 6%
0.6%
24
The Plant Genome
○ 57 papers in training set
Top 1%
0.6%
25
Life Science Alliance
● 285 papers in training set
Top 10%
0.6%

"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.