Wsc1 acts as a piezosensor in Saccharomyces cerevisiae, enhancing glycerol efflux via aquaglyceroporin Fps1 in response to high hydrostatic pressure
Mochizuki, T.; Tanigawa, T.; Shindo, S.; Suematsu, M.; Oguchi, Y.; Mioka, T.; Kato, Y.; Fujiyama, M.; Hatano, E.; Yamaguchi, M.; Chibana, H.; Abe, F.
Show abstract
The fungal cell wall is the initial barrier for the fungi against diverse external stresses, such as osmolarity changes, harmful drugs, and mechanical injuries. This study explores the roles of osmoregulation and the cell wall integrity (CWI) pathway in response to high hydrostatic pressure in the yeast Saccharomyces cerevisiae. We demonstrate the roles of the transmembrane mechanosensor Wsc1 and aquaglyceroporin Fps1 in a general mechanism to maintain cell growth under high-pressure regimes. The promotion of water influx into cells at 25 MPa, as evident by an increase in cell volume and a loss of the plasma membrane eisosome structure, promotes the activation of Wsc1, an activator of the CWI pathway. The downstream mitogen-activated protein kinase Slt2 was hyperphosphorylated at 25 MPa. Glycerol efflux increases via Fps1 phosphorylation, which is initiated by downstream components of the CWI pathway and contributes to the reduction in intracellular osmolarity under high pressure. The elucidation of the mechanisms underlying adaption to high pressure through the well-established CWI pathway could potentially translate to mammalian cells and provide novel insights into cellular mechanosensation.
Matching journals
The top 5 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
Similar papers in this journal
- High and stable ATP levels prevent aberrant intracellular protein aggregation 92%
- Growth-dependent signals drive an increase in early G1 cyclin concentration to link cell cycle entry with cell growth 92%
- Acidocalcisome-like vacuoles constitute a feedback-controlled phosphate buffering system for the cytosol 92%
Similar papers in this journal
Similar papers in this journal
- Osmolarity regulates C. elegans egg-laying behavior via parallel chemosensory and biophysical mechanisms 90%
- Differential tissue stiffness of body column facilitates locomotion of Hydra on solid substrates 89%
- Responses of the in vitro turtle brain to visual and auditory stimuli during severe hypoxia 89%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.