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Two-photon targeted, quad whole-cell patch clamping robot

Gonzalez, G. I. V.; Kgwarae, P. O.; Schultz, S. R.

2022-11-16 bioengineering Community evaluation
10.1101/2022.11.14.516499 bioRxiv
Show abstract

We present an automated quad-channel patch-clamp technology platform for ex vivo brain slice electrophysiology, capable of both blind and two-photon targeted robotically automated patching. The robot scales up the patch-clamp singlecell recording technique to four simultaneous channels, with seal success rates for two-photon targeted and blind modes of 54% and 68% respectively. In 50% of targeted trials (where specific cells were required), at least 2 simultaneous recordings were obtained. For blind mode, most trials yielded dual or triple recordings. This robot, a milestone on the path to a true in vivo robotic multi-patching technology platform, will allow numerous studies into the function and connectivity patterns of both primary and secondary cell types.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.