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Ex vivo intestinal permeability assay (X-IPA) for tracking barrier function dynamics

Bootz-Maoz, H.; Simon, A.; Del Mare-Roumani, S.; Bennet, Y.; Zheng, D.; Amidror, S.; Elinav, E.; Yissachar, N.

2022-11-08 microbiology
10.1101/2022.11.08.515584 bioRxiv
Show abstract

The intestinal epithelial barrier facilitates homeostatic host-microbiota interactions and immunological tolerance. However, mechanistic dissections of barrier dynamics following luminal stimulation pose a substantial challenge. Here, we describe an ex-vivo intestinal permeability assay, X-IPA, for quantitative analysis of gut permeability dynamics at the whole-tissue level. We demonstrate that specific gut microbes and metabolites induce rapid, dose-dependent increases to gut permeability, thus providing a powerful approach for precise investigation of barrier functions.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.