Identification of key residues of the DNA glycosylase OGG1 controlling efficient DNA scanning and recruitment to oxidized bases in living cells
D'Augustin, O.; Gaudon, V.; Siberchicot, C.; Smith, R.; Chapuis, C.; DEPAGNE, J.; Veaute, X.; BUSSO, D.; Di-Guilmi, A.-M.; Castaing, B.; Radicella, J. P.; Campalans, A.; Huet, S.
Show abstract
The DNA-glycosylase OGG1 oversees the detection and clearance of the 7,8-dihydro-8-oxoguanine (8-oxoG), which is the most frequent form of oxidized base in the genome. This lesion is deeply buried within the double-helix and its detection requires careful inspection of the bases by OGG1 via a mechanism that remains only partially understood. By analyzing OGG1 dynamics in the nucleus of living human cells, we demonstrate that the glycosylase constantly scans the DNA by rapidly alternating between diffusion within the nucleoplasm and short transits on the DNA. This scanning process, that we find to be tightly regulated by the conserved residue G245, is crucial for the rapid recruitment of OGG1 at oxidative lesions induced by laser micro-irradiation. Furthermore, we show that residues Y203, N149 and N150, while being all involved in early stages of 8-oxoG probing by OGG1 based on previous structural data, differentially regulate the scanning of the DNA and recruitment to oxidative lesions.
Matching journals
The top 2 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Recovery of protein synthesis to measure transcription-coupled DNA repair in living cells and tissues 96%
- Tethering-facilitated DNA 'opening' and complementary roles of β-hairpin motifs in the Rad4/XPC DNA damage sensor protein 96%
- Uncovering nick DNA binding by LIG1 at the single-molecule level 96%
Similar papers in this journal
- Topological stress triggers persistent DNA lesions in ribosomal DNA with ensuing formation of PML-nucleolar compartment 95%
- Topoisomerase VI is a chirally-selective, preferential DNA decatenase 95%
- Systematic analysis of the molecular and biophysical properties of key DNA damage response factors 95%
Similar papers in this journal
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.