An improved Erk biosensor reveals oscillatory Erk dynamics driven by mitotic erasure during early development
Wilcockson, S. G.; Guglielmi, L.; Araguas Rodriguez, P.; Amoyel, M.; Hill, C. S.
10.1101/2022.11.03.515001 bioRxivShow abstract
Erk signaling dynamics elicit distinct cellular responses in a variety of contexts. The early zebrafish embryo is an ideal model to explore the role of Erk signaling dynamics in vivo, as a gradient of activated diphosphorylated Erk (P-Erk) is induced by Fgf signaling at the blastula embryonic margin. Here we describe an improved Erk-specific biosensor which we term modified Erk Kinase Translocation Reporter (modErk-KTR). We demonstrate the utility of this biosensor in vitro and in developing zebrafish and Drosophila embryos. Moreover, we show that Fgf/Erk signaling is dynamic and coupled to tissue growth during both early zebrafish and Drosophila development. Signaling is rapidly extinguished just prior to mitosis, which we refer to as mitotic erasure, inducing periods of inactivity, thus providing a source of heterogeneity in an asynchronously dividing tissue. Our modified reporter and transgenic lines represent an important resource for interrogating the role of Erk signaling dynamics in vivo.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Bicc1 and dicer regulate left-right patterning through posttranscriptionalcontrol of the Nodal-inhibitor dand5 97%
- The ubiquitin ligase Hecw controls oogenesis and neuronal homeostasis by promoting the liquid state of ribonucleoprotein particles 96%
- The deubiquitylating enzyme Fat Facets promotes Fat signalling and restricts tissue growth 96%
Similar papers in this journal
Similar papers in this journal
- Optogenetic control of the Bicoid morphogen reveals fast and slow modes of gap gene regulation 98%
- Thalamic regulation of a visual critical period and motor behavior 97%
- BMP-dependent patterning of ectoderm tissue material properties modulates lateral mesendoderm cell migration during early zebrafish gastrulation 96%
Similar papers in this journal
- Tissue mechanics and systemic signaling safeguard epithelial tissue against spindle misorientation. 96%
- Combined inactivation of RB and Hippo pathways converts differentiating photoreceptors into eye progenitor cells through derepression of homothorax 96%
- Microtubules coordinate mitochondria transport with myofibril morphogenesis during muscle development 95%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.