Paradox Found: Global Accounting of Lymphocyte Protein Synthesis
Seedhom, M.; Dersh, D.; Holly, J.; Pavon-Eternod, M.; Wei, J.; Shores, L.; David, A.; Santos, J.; Hickman, H.; Yewdell, J.
Show abstract
Rapid lymphocyte cell division places enormous demands on the protein synthesis machinery. Flow cytometric measurement of puromycylated ribosome-associated nascent chains after treating cells or mice with translation initiation inhibitors reveals that ribosomes in resting lymphocytes in vitro and in vivo elongate at typical rates for mammalian cells. Intriguingly, elongation rates can be increased up to 30% by activation in vivo or fever temperature in vitro. Resting and activated lymphocytes possess abundant monosome populations, most of which actively translate in vivo, while in vitro, nearly all can be stalled prior to activation. Quantitating lymphocyte protein mass and ribosome count reveals a paradoxically high ratio of cellular protein to ribosomes insufficient to support their rapid in vivo division, suggesting that the activated lymphocyte proteome in vivo may be generated in an unusual manner. Our findings demonstrate the importance of a global understanding of protein synthesis in lymphocytes and other rapidly dividing immune cells.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Dual Ribosome Profiling reveals metabolic limitations of cancer and stromal cells in thetumor microenvironment 96%
- Functionally distinct roles for eEF2K in the control of ribosome availability and p-body abundance in sensory neurons 96%
- Cap-dependent translation initiation monitored in living cells 96%
Similar papers in this journal
- Multi-omics and biochemical reconstitution reveal CDK7-dependent mechanisms controlling RNA polymerase II function at gene 5'- and 3'-ends 95%
- Conserved heterodimeric GTPase Rbg1/Tma46 promotes efficient translation in eukaryotic cells 95%
- Genome-wide CRISPR screens identify noncanonical translation factor eIF2A as an enhancer of SARS-CoV-2 programmed -1 ribosomal frameshifting 95%
Similar papers in this journal
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.