Within-animal comparison of microdialysis and fiber photometry in amphetamine-exposed mice
Ejdrup, A. L.; Wellbourne-Wood, J.; Dreyer, J. K.; Guldhammer, N.; Lycas, M. D.; Gether, U.; Hall, B. J.; Soerensen, G.
Show abstract
A fundamental concept in neuroscience is the transmission of information between neurons via neurotransmitters, -modulators, and - peptides. For the past decades the gold standard for measuring neurochemicals in awake animals has been microdialysis (MD). The emergence of genetically encoded fluorescence-based biosensors, as well as in vivo optical techniques such as fiber photometry (FP), have introduced technologically distinct means of measuring neurotransmission. To directly compare MD and FP, we performed concurrent within-animal recordings of extracellular dopamine (DA) in the dorsal striatum (DS) before and after administration of amphetamine in awake, freely behaving mice expressing the dopamine sensor dLight1.3b. We show that despite temporal differences, MD- and FP-based readouts of DA correlate well within mice. Down-sampling of FP data showed temporal correlation to MD data, with less variance observed using FP. We also present evidence that DA fluctuations periodically reach low levels and naive animals have rapid, pre-drug DA dynamics measured with FP that correlate to the subsequent pharmacodynamics of amphetamine as measured with MD and FP.
Matching journals
The top 6 journals account for 50% of the predicted probability mass.
Similar papers in this journal
- Sub-second striatal dopamine dynamics assessed by simultaneous fast-scan cyclic voltammetry and fluorescence biosensor 98%
- Synaptotagmin-1-dependent phasic axonal dopamine release is dispensable for basic motor behaviors in mice. 95%
- Development of a genetically-encoded sensor for probing endogenous nociceptin opioid peptide release 94%
Similar papers in this journal
Similar papers in this journal
- Dopamine transmission at D1 and D2 receptors in the nucleus accumbens contributes to the expression of incubation of cocaine craving 94%
- Selective Nigrostriatal Dopamine Excess Impairs Behaviors Linked to the Cognitive and Negative Symptoms of Psychosis 94%
- Operant self-stimulation of thalamic terminals in the dorsomedial striatum is constrained by metabotropic glutamate receptor 2 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.