Adult Drosophila legs do not regenerate after amputation
Sustar, A.; Tuthill, J. C.
Show abstract
A recent paper by Abrams et al. (2021) claimed that a simple dietary supplement is sufficient to induce appendage regeneration in jellyfish, flies, and mice. This would be remarkable, if true, because it was previously thought that flies and mice lack the capacity for regeneration after injury. We therefore sought to replicate their provocative results. We amputated one tibia of over 1000 fruit flies, fed them control or supplemented diets, and carefully examined their legs three weeks post-injury. We did not, however, observe any instances of leg regeneration. We conducted additional experiments that confirmed the complete absence of neurons, muscles, or other living cells in amputated tibias. Abrams et al. also reported the formation of a white blob at the amputation site, which they interpreted as an intermediate regeneration morphology. We tested this hypothesis more rigorously and conclude that the white blob consists of bacteria. Overall, we failed to find any evidence for leg regeneration in Drosophila, even when flies were fed the supplemented diet. Our results therefore contradict the overarching conclusion of Abrams et al. that dietary supplements are sufficient to unlock an ancestral mechanism that induces appendage regeneration.
Matching journals
The top 7 journals account for 50% of the predicted probability mass.
Similar papers in this journal
Similar papers in this journal
- Evolutionary conservation of midline axon guidance activity between Drosophila and Tribolium Frazzled 95%
- Wnt/β-catenin signaling promotes posterior axial regeneration in non-regenerative tissue of the annelid Capitella teleta 94%
- Role of BMP signaling during early development of the annelid Capitella teleta 93%
Similar papers in this journal
- The Drosophila melanogaster enzyme glycerol-3-phosphate dehydrogenase 1 is required for oogenesis, embryonic development, and amino acid homeostasis 94%
- Transgenic Drosophila lines for LexA-dependent gene and growth regulation 94%
- Pvr and downstream signaling factors are required for spreading of Drosophila hemocytes at larval wound sites 94%
Similar papers in this journal
- The embryonic role of juvenile hormone in the firebrat, Thermobia domestica, reveals its function before its involvement in metamorphosis 94%
- Auxin Exposure Disrupts Feeding Behavior and Fatty Acid Metabolism in Adult Drosophila 94%
- Unc-4 acts to promote neuronal identity and development of the take-off circuit in the Drosophila CNS 93%
"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.