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High-throughput deep tissue two-photon microscopy at kilohertz frame rates

Xiao, S.; Giblin, J.; Boas, D. A.; Mertz, J.

2022-10-25 bioengineering
10.1101/2022.10.24.513414 bioRxiv
Show abstract

Strategies to image biological phenomena at millisecond time scales are generally technically challenging and suffer from compromises between imaging field-of-view, depth penetration and excitation efficiency in thick tissue. We present a simple and cost-effective solution that enables a conventional video-rate two-photon microscope (2PM) to perform 2D scanning at kilohertz frame rates, while preserving all the benefits of standard 2PM, which we demonstrate by imaging neurovascular dynamics in mouse brains.

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