Single-fibre proteomics reveals a greater fibre-type specific mitochondrial remodelling with moderate than high-intensity training.
Reisman, E.; Botella Ruiz, J.; Huang, C.; Schittenhelm, R. B.; Stroud, D. A.; Granata, C.; Chandrasiri, S.; Ramm, G.; Oorschot, V.; Caruana, N. J.; Bishop, D. J.
Show abstract
Analyses of mitochondrial adaptations in human skeletal muscle have mostly used whole-muscle samples, where results may be confounded by the presence of a mixture of type I and II muscle fibres. Using our adapted mass spectrometry-based proteomics workflow, we provide new insights into fibre-specific mitochondrial differences in human skeletal muscle before and after training. Our findings challenge previous conclusions regarding the extent of fibre-type-specific remodelling of the mitochondrial proteome and highlight that most baseline differences in mitochondrial protein abundances between fibre types reported by us, and others, might be due to differences in total mitochondrial content or a consequence of adaptations to habitual physical activity (or inactivity). Most training-induced changes in different mitochondrial functional groups, in both fibre types, were stoichiometrically linked to changes in markers of mitochondrial content.
Matching journals
The top 4 journals account for 50% of the predicted probability mass.