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Hippocampal inactivation during rearing on hind legs impairs spatial memory

Layfield, D.; Sidell, N.; Blankenberger, K.; Newman, E. L.

2022-10-21 neuroscience
10.1101/2022.10.21.513207 bioRxiv
Show abstract

Spatial memory requires an intact hippocampus. Hippocampal function during epochs of locomotion and quiet rest (e.g., grooming and reward consumption) have been the target of extensive study. However, during navigation rats frequently rear up on to their hind legs and the importance of hippocampal activity during these periods of attentive sampling for spatial memory is unknown. To address this, we tested the necessity of dorsal hippocampal activity during rearing epochs in the study phase of a delayed win-shift task for memory performance in the subsequent test phase. Hippocampal activity was manipulated with closed-loop, bilateral, optogenetic inactivation. Spatial memory accuracy was significantly and selectively reduced when the dorsal hippocampus was inactivated during rearing epochs at encoding. These data show that hippocampal activity during periods of rearing can be important for spatial memory, revealing a novel link between hippocampal function during epochs of rearing and spatial memory.

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