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Biosynthesis of the Multifunctional Isopropylstilbene in Photorhabdus laumondii Involves Cross-Talk between Specialized and Primary Metabolism

Kavakli, S.; Grammbitter, G. L. C.; Bode, H. B.

2022-10-21 microbiology
10.1101/2022.10.20.513026 bioRxiv
Show abstract

Isopropylstilbene (IPS) derived from the entomopathogenic bacterium Photorhabdus represents the only known stilbene which is not produced by a plant stilbene synthase but a bacterial PKS II synthase. While the exclusive cyclization reaction, responsible for the formation of the characteristic iso-branched side-chain of the molecule, was studied in the past, some parts of the biosynthetic route remained elusive. In this study, we revealed the role of StlB that is able to produce CoA-derivatives and demonstrated the elongation of cinnamoyl-CoA with enzymes from the bacterial fatty acid biosynthesis pathway. Thus, we deciphered cross-talk between the enzymes from primary and specialized metabolism. These insights led, for the first time, to the production of IPS in a heterologous host.

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"Similar papers" are the closest papers from that journal in the model's embedding space. They show what the match is built on, but the ranking comes mostly from a classifier over the whole training set, not from these examples alone.