Genome sequencing of the Monkeypox virus 2022 outbreak with Amplicon-based Oxford Nanopore MinION sequencing
Brinkmann, A.; Pape, K.; Uddin, S.; Woelk, N.; Foerster, S.; Jessen, H.; Michel, J.; Kohl, C.; Schaade, L.; Nitsche, A.
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We present an amplicon-based assay for MinION Nanopore sequencing of monkeypox virus genomes from clinical specimens, obtaining high-quality results for up to 99 % of the genomes, even for samples with high Ct values of up to 30 which are not suitable for shotgun sequencing.
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